Quantibody® array is an array-based multiplex sandwich ELISA system for simultaneous quantitative measurement the concentration of multiple cytokines in serum, plasma, CSF, cell lysate, conditioned media and other body fluid. It combines the advantages of the high detection specificity/ sensitivity of ELISA and the high throughput of the arrays. Like a traditional sandwich-based ELISA, it uses a pair of cytokine-specific antibodies for detection. A capture antibody is first bound to the glass surface. After incubation with the sample, the target cytokine is trapped on the solid surface. A second biotin-labeled detection antibody is then added, which can recognize a different isotope of the target cytokine. The cytokine-antibody-biotin complex can then be visualized through the addition of the streptavidin-labeled Fluorescent dye using a laser scanner. Unlike the traditional ELISA, Quantibody products use array format. By arraying multiple cytokine specific capture antibodies onto a glass support, multiplex detection of cytokines in one experiment is made possible.
KPL’s new DyLightTM fluorescent conjugates offer superior performance over conventional Cy dyes and fluorescein and comparable brightness and photostability to Alexa Fluor dyes. They combine the sensitivity and reproducibility of KPL’s affinity purified antibodies and streptavidin with a series of intense fluorescent DyLight dyes for brilliant analysis in fluorescence microscopy, flow cytometry, Western blotting, ELISA and other fluorescence applications. KPL offers DyLight conjugates with three DyLight dyes (DyLight 488, 549, 649) with well-differentiated emission spectra ideal for sensitive multi-color analysis.